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rabbit anti mouse ckit  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rabbit anti mouse ckit
    Figure 1. Receptors for SCF and G-CSF are expressed on brain endothelial cells. (A,B) Representative 3-dimensional (3D) confocal images. Capillaries (CD31+, red) in the cortex of the adult mouse brain show co-expression with the receptors for SCF (A) <t>(Ckit,</t> green) and G-CSF (B) (GCSFR, green). Scale bar, 10 µm. (C,D) Representative flow cytometry data. The expressions of Ckit (C) and GCSFR (D) on cerebral endothelial cells are detected by flow cytometry analysis of CD45−CD31+ gated endothelial cells isolated from adult mouse brain. Mean fluorescence intensity (MFI) of Ckit (C) and GCSFR (D) expressing on cerebral endothelial cells is presented in flow cytometry histograms. Isotype: isotype control antibody. Repeated experiments, n = 4. (E,F) Representative confocal images of immunofluorescence staining. The expression of Ckit (E) (red) and GCSFR (F) (red) are seen on bEnd.3 endothelial cells (CD31+, green). Scale bar, 50 µm. Repeated experiments, n = 3. (G,H) Representative flow cytometry histograms showing the expression of Ckit (G) and GCSFR (H) on bEnd.3 endothelial cells. MFI: Mean fluorescence intensity. Isotype: isotype control antibody. Repeated experiments, n = 5.
    Rabbit Anti Mouse Ckit, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 3859 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse ckit/product/Santa Cruz Biotechnology
    Average 96 stars, based on 3859 article reviews
    rabbit anti mouse ckit - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "Hematopoietic Growth Factors Regulate the Entry of Monocytes into the Adult Brain via Chemokine Receptor CCR5."

    Article Title: Hematopoietic Growth Factors Regulate the Entry of Monocytes into the Adult Brain via Chemokine Receptor CCR5.

    Journal: International journal of molecular sciences

    doi: 10.3390/ijms25168898

    Figure 1. Receptors for SCF and G-CSF are expressed on brain endothelial cells. (A,B) Representative 3-dimensional (3D) confocal images. Capillaries (CD31+, red) in the cortex of the adult mouse brain show co-expression with the receptors for SCF (A) (Ckit, green) and G-CSF (B) (GCSFR, green). Scale bar, 10 µm. (C,D) Representative flow cytometry data. The expressions of Ckit (C) and GCSFR (D) on cerebral endothelial cells are detected by flow cytometry analysis of CD45−CD31+ gated endothelial cells isolated from adult mouse brain. Mean fluorescence intensity (MFI) of Ckit (C) and GCSFR (D) expressing on cerebral endothelial cells is presented in flow cytometry histograms. Isotype: isotype control antibody. Repeated experiments, n = 4. (E,F) Representative confocal images of immunofluorescence staining. The expression of Ckit (E) (red) and GCSFR (F) (red) are seen on bEnd.3 endothelial cells (CD31+, green). Scale bar, 50 µm. Repeated experiments, n = 3. (G,H) Representative flow cytometry histograms showing the expression of Ckit (G) and GCSFR (H) on bEnd.3 endothelial cells. MFI: Mean fluorescence intensity. Isotype: isotype control antibody. Repeated experiments, n = 5.
    Figure Legend Snippet: Figure 1. Receptors for SCF and G-CSF are expressed on brain endothelial cells. (A,B) Representative 3-dimensional (3D) confocal images. Capillaries (CD31+, red) in the cortex of the adult mouse brain show co-expression with the receptors for SCF (A) (Ckit, green) and G-CSF (B) (GCSFR, green). Scale bar, 10 µm. (C,D) Representative flow cytometry data. The expressions of Ckit (C) and GCSFR (D) on cerebral endothelial cells are detected by flow cytometry analysis of CD45−CD31+ gated endothelial cells isolated from adult mouse brain. Mean fluorescence intensity (MFI) of Ckit (C) and GCSFR (D) expressing on cerebral endothelial cells is presented in flow cytometry histograms. Isotype: isotype control antibody. Repeated experiments, n = 4. (E,F) Representative confocal images of immunofluorescence staining. The expression of Ckit (E) (red) and GCSFR (F) (red) are seen on bEnd.3 endothelial cells (CD31+, green). Scale bar, 50 µm. Repeated experiments, n = 3. (G,H) Representative flow cytometry histograms showing the expression of Ckit (G) and GCSFR (H) on bEnd.3 endothelial cells. MFI: Mean fluorescence intensity. Isotype: isotype control antibody. Repeated experiments, n = 5.

    Techniques Used: Expressing, Flow Cytometry, Isolation, Fluorescence, Control, Immunofluorescence, Staining



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    Figure 1. Receptors for SCF and G-CSF are expressed on brain endothelial cells. (A,B) Representative 3-dimensional (3D) confocal images. Capillaries (CD31+, red) in the cortex of the adult mouse brain show co-expression with the receptors for SCF (A) <t>(Ckit,</t> green) and G-CSF (B) (GCSFR, green). Scale bar, 10 µm. (C,D) Representative flow cytometry data. The expressions of Ckit (C) and GCSFR (D) on cerebral endothelial cells are detected by flow cytometry analysis of CD45−CD31+ gated endothelial cells isolated from adult mouse brain. Mean fluorescence intensity (MFI) of Ckit (C) and GCSFR (D) expressing on cerebral endothelial cells is presented in flow cytometry histograms. Isotype: isotype control antibody. Repeated experiments, n = 4. (E,F) Representative confocal images of immunofluorescence staining. The expression of Ckit (E) (red) and GCSFR (F) (red) are seen on bEnd.3 endothelial cells (CD31+, green). Scale bar, 50 µm. Repeated experiments, n = 3. (G,H) Representative flow cytometry histograms showing the expression of Ckit (G) and GCSFR (H) on bEnd.3 endothelial cells. MFI: Mean fluorescence intensity. Isotype: isotype control antibody. Repeated experiments, n = 5.
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    Figure 1. Receptors for SCF and G-CSF are expressed on brain endothelial cells. (A,B) Representative 3-dimensional (3D) confocal images. Capillaries (CD31+, red) in the cortex of the adult mouse brain show co-expression with the receptors for SCF (A) <t>(Ckit,</t> green) and G-CSF (B) (GCSFR, green). Scale bar, 10 µm. (C,D) Representative flow cytometry data. The expressions of Ckit (C) and GCSFR (D) on cerebral endothelial cells are detected by flow cytometry analysis of CD45−CD31+ gated endothelial cells isolated from adult mouse brain. Mean fluorescence intensity (MFI) of Ckit (C) and GCSFR (D) expressing on cerebral endothelial cells is presented in flow cytometry histograms. Isotype: isotype control antibody. Repeated experiments, n = 4. (E,F) Representative confocal images of immunofluorescence staining. The expression of Ckit (E) (red) and GCSFR (F) (red) are seen on bEnd.3 endothelial cells (CD31+, green). Scale bar, 50 µm. Repeated experiments, n = 3. (G,H) Representative flow cytometry histograms showing the expression of Ckit (G) and GCSFR (H) on bEnd.3 endothelial cells. MFI: Mean fluorescence intensity. Isotype: isotype control antibody. Repeated experiments, n = 5.
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    Figure 1. Receptors for SCF and G-CSF are expressed on brain endothelial cells. (A,B) Representative 3-dimensional (3D) confocal images. Capillaries (CD31+, red) in the cortex of the adult mouse brain show co-expression with the receptors for SCF (A) <t>(Ckit,</t> green) and G-CSF (B) (GCSFR, green). Scale bar, 10 µm. (C,D) Representative flow cytometry data. The expressions of Ckit (C) and GCSFR (D) on cerebral endothelial cells are detected by flow cytometry analysis of CD45−CD31+ gated endothelial cells isolated from adult mouse brain. Mean fluorescence intensity (MFI) of Ckit (C) and GCSFR (D) expressing on cerebral endothelial cells is presented in flow cytometry histograms. Isotype: isotype control antibody. Repeated experiments, n = 4. (E,F) Representative confocal images of immunofluorescence staining. The expression of Ckit (E) (red) and GCSFR (F) (red) are seen on bEnd.3 endothelial cells (CD31+, green). Scale bar, 50 µm. Repeated experiments, n = 3. (G,H) Representative flow cytometry histograms showing the expression of Ckit (G) and GCSFR (H) on bEnd.3 endothelial cells. MFI: Mean fluorescence intensity. Isotype: isotype control antibody. Repeated experiments, n = 5.
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    Image Search Results


    Figure 1. Receptors for SCF and G-CSF are expressed on brain endothelial cells. (A,B) Representative 3-dimensional (3D) confocal images. Capillaries (CD31+, red) in the cortex of the adult mouse brain show co-expression with the receptors for SCF (A) (Ckit, green) and G-CSF (B) (GCSFR, green). Scale bar, 10 µm. (C,D) Representative flow cytometry data. The expressions of Ckit (C) and GCSFR (D) on cerebral endothelial cells are detected by flow cytometry analysis of CD45−CD31+ gated endothelial cells isolated from adult mouse brain. Mean fluorescence intensity (MFI) of Ckit (C) and GCSFR (D) expressing on cerebral endothelial cells is presented in flow cytometry histograms. Isotype: isotype control antibody. Repeated experiments, n = 4. (E,F) Representative confocal images of immunofluorescence staining. The expression of Ckit (E) (red) and GCSFR (F) (red) are seen on bEnd.3 endothelial cells (CD31+, green). Scale bar, 50 µm. Repeated experiments, n = 3. (G,H) Representative flow cytometry histograms showing the expression of Ckit (G) and GCSFR (H) on bEnd.3 endothelial cells. MFI: Mean fluorescence intensity. Isotype: isotype control antibody. Repeated experiments, n = 5.

    Journal: International journal of molecular sciences

    Article Title: Hematopoietic Growth Factors Regulate the Entry of Monocytes into the Adult Brain via Chemokine Receptor CCR5.

    doi: 10.3390/ijms25168898

    Figure Lengend Snippet: Figure 1. Receptors for SCF and G-CSF are expressed on brain endothelial cells. (A,B) Representative 3-dimensional (3D) confocal images. Capillaries (CD31+, red) in the cortex of the adult mouse brain show co-expression with the receptors for SCF (A) (Ckit, green) and G-CSF (B) (GCSFR, green). Scale bar, 10 µm. (C,D) Representative flow cytometry data. The expressions of Ckit (C) and GCSFR (D) on cerebral endothelial cells are detected by flow cytometry analysis of CD45−CD31+ gated endothelial cells isolated from adult mouse brain. Mean fluorescence intensity (MFI) of Ckit (C) and GCSFR (D) expressing on cerebral endothelial cells is presented in flow cytometry histograms. Isotype: isotype control antibody. Repeated experiments, n = 4. (E,F) Representative confocal images of immunofluorescence staining. The expression of Ckit (E) (red) and GCSFR (F) (red) are seen on bEnd.3 endothelial cells (CD31+, green). Scale bar, 50 µm. Repeated experiments, n = 3. (G,H) Representative flow cytometry histograms showing the expression of Ckit (G) and GCSFR (H) on bEnd.3 endothelial cells. MFI: Mean fluorescence intensity. Isotype: isotype control antibody. Repeated experiments, n = 5.

    Article Snippet: Anti-mouse antibodies used for flow cytometry included: rat anti-CD31 (MEC 13.3, BD Pharmingen, San Diego, CA, USA), rat anti-CD45-APC (30F11, eBioscience, San Diego, CA, USA), rabbit anti-mouse ckit (sc-168, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-mouse GCSFR (sc-694, Santa Cruz Biotechnology, USA), rabbit IgG (sc-3888, Santa Cruz Biotechnology, USA), anti-CCR5-PE (HM-CCR5 (7A4), eBioscience, USA), antiCD106-FITC (VCAM-1, 429, eBioscience, USA), and anti-CD54-FITC (ICAM-1, YN1/1.7.4, eBioscience, USA).

    Techniques: Expressing, Flow Cytometry, Isolation, Fluorescence, Control, Immunofluorescence, Staining